Aim - to evaluate the viability of leukemic cells sensitive (L1210S) and resistant (L1210R) to cisplatin, ROS production and free cytosolic Ca<^>2+ concentration under treatment with cisplatin or its combination with photoexcited fullerene C60. Cell viability was assessed by the MTT reduction assay. Light-emitting diode lamp (2,45 J/cm<^>2) was used for photoexcitation of intracellular accumulated fullerene C60. Free cytosolic calcium concentration ([Ca<^>2+]i) and ROS production in cells were estimated with the use of fluorescent probes Indo-1 and 2',7'-dichlorodihydrofluorescein diacetate (DCF-DA), respectively. It is shown, that viability of L1210R cells wasn't changed under treatment with cisplatin in concentration range 0,1 - 10 mu g/ml. 50 % and 30 % decrease of L1210S cells were observed after 24 h of incubation with cisplatin at concentrations 5 and 1 mu g/ml, respectively. Intensification of extranuclear cytotoxic effects (ROS production and [Ca<^>2+]i increase) after treatment with 1 mu g/ml was detected in L1210S, but not in L1210R cells. The most strongly pronounced increase of ROS production and [Ca<^>2+]i in both L1210 cell lines was revealed in dynamics after combined treatment with cisplatin (1 mu g/ml) and photoexcited fullerene C60 (10<^>-5 M) and was followed by decreased viability of not only L1210S, but of L1210R cells as well. Conclusion: combined treatment with photoexcited C60 and cisplatin allowed to decrease effective concentration of cisplatin against parental LI 210 cells and to increase sensibility of resistant cells to the drug.

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• Прилуцька Світлана Володимирівна (біологічні науки)

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