Paryzhak S. Ya. Simple two-step covalent protein conjugation to PEG-coated nanocrystals / S. Ya. Paryzhak, T. I. Dumych, O. I. Karmash, E. E. Bila, D. Stachowiak, M. Banski, A. Podhorodecki, R. O. Bilyy // The Ukr. Biochem. J.. - 2018. - 90, № 2. - С. 8-12. - Бібліогр.: 12 назв. - англ.Covering of nanocrystals (NC) with a polyethylene glycol (PEG) envelop is a common way to increase their hydrophilicity, and compatibility with bio-systems, including increased retention time in the body. Colloidal semiconductor NC, also known as quantum dots (QD), particularly benefit from covering with PEG due to passivation of the inorganic core, while maintaining physical properties of the core. Despite many advantages of covering the surface with PEG, the covalent attachment of protein to hydroxyls of PEG is complicated. Here we propose a simple two-step approach for modification of PEG residues with subsequent covalent attachment of proteins. We were able to achieve specific NC targeting by means of attached protein as well as preserve their optical parameters (fluorescence intensity) in chemical reaction conditions. In the optimized protocol, ensuring removal of chemical byproducts by dialysis, we were able to omit the need for centrifugation (usually a limiting step due to particle size). The obtained NC-protein conjugate solutions contained 0,25x of initial unmodified NC amount, ensuring a low dilution of the sample. During all reactions the pH range was optimized to be between 6 to 8. The proposed approach can be easily modified for covalent targeting of different PEG-covered nanocomposites with proteins. Індекс рубрикатора НБУВ: Г596
Рубрики:
Шифр НБУВ: Ж21341/а Пошук видання у каталогах НБУВ
Повний текст Наукова періодика України Додаткова інформація про автора(ів) публікації: (cписок формується автоматично, до списку можуть бути включені персоналії з подібними іменами або однофамільці) ![](/irbis_nbuv/images/info.png) Якщо, ви не знайшли інформацію про автора(ів) публікації, маєте бажання виправити або відобразити більш докладну інформацію про науковців України запрошуємо заповнити "Анкету науковця"
|